Two ANA nuclear staining patterns are of particular clinical importance in the context of liver disease, i.e. the rim-like (also referred to as membranous) and the multiple-nuclear dot patterns (Fig. 2D, E), which, in association with a cholestatic biochemical profile, are characteristic of PBC, being particularly helpful in the diagnosis of AMA-negative PBC [12]. This evidence concerns the gene BTG3 and liver disorder.