The 22 hub genes (ASPM, AURKA, CCNA2, CCBN2, CDC20, CDKN3, CENPF, FEN1, FOXM1, HMMR, KIF20A, MCM4, MELK, NCAPG, NUSAP1, OIP5, PRC1, PTTG1, TOP2A, TPX2, TRIP13 and UBE2C) were up-regulated n HCC tissues comparing normal liver tissues (Figure 3). This evidence concerns the gene CDC20 and hepatocellular carcinoma.