Moreover, the deletions of PTS1 and pmi did not enhance the resistance of E. faecalis to ROS or lysozyme (data not shown), and infections with these two strains did not clearly attenuate the acidification of phagosomes (Fig. S4B), the levels of ROS (Fig. S4C), or the activities of cathepsin B (Fig. S4D) in RAW264.7 cells compared with WT infection. This evidence concerns the gene CTSB and infection.