Wnt signaling regulation has been connected to Six1, Six2, Six3, Six4, and Six6. Six1 overexpression upregulated Wnt pathway genes and promoted β-catenin nuclear localization in mammary gland tumors and colorectal cancer cell lines (McCoy et al., 2009; Song et al., 2019). The gene discussed is SIX4; the disease is colorectal cancer.