This panel enabled simultaneous evaluation of tumor, immune, and stroma biomarkers in a single slide, visualizing the opposite distribution of HIF1α and TIMP1 in the intratumor and invasive margins, respectively, (Figures 5A, B) and accumulation of immune markers (CD3 and CD68) and stroma markers (aSMA) in adjacent non-malignant regions (Figure 5C and Supplementary Figure 5). This evidence concerns the gene HIF1A and neoplasm.