REN and hypertensive disorder: In this study, chronic infusion with angiotensin II via an osmotic mini pump was able to induce hypertension in middle-aged mice with the mechanism by which the renin-angiotensin system (RAS) regulates blood pressure in the human body (Yang and Xu, 2017), and the results were consistent with those of previous publications (Gentile et al., 2009; Chan et al., 2012; Lu et al., 2015).