LGR6 and cervical carcinoma: In further studies, western blot and RT-PCR demonstrated that overexpression of TCF7L2 upregulated LGR6 expression in cervical cancer cells at both the protein and mRNA levels (Fig. 6E–G), implying that TCF7L2 and β-catenin (CTNNB1) are upstream regulators of LGR6.