We observed that activating septal OXTr neural projections with intra-HDB J60 injections induced anxiety, as indicated by the decreased time spent in the center (Fig. 4b–c), percent distance traveled in the center (Fig. 4d), and number of center entries (Fig. 4e) of the open field in the OXTr neuron hM3Dq-transduced mice compared to the OXTr neuron mCherry-transduced mice. This evidence concerns the gene OXTR and Anxiety.