Our study shows the cells grown in 3D-GMH with serum overexpress and secrete CXCL1, CXCL5, IGFBP2, PTX3, THBS1, VEGFA, and VCAM1—all genes expressed in perivascular or perinecrotic zone of glioblastoma tissues that are hotspots for immune cells5. This evidence concerns the gene PTX3 and glioblastoma.