By analyzing the differences in epithelial expression program scores and TME cell-type proportions associated with TP53 or NOTCH1 mutation status, we found that ESCC tumors with the mutant TP53 had higher Cycling and Epi1 program scores and higher immune suppression levels than tumors with the wild-type TP53 (Fig. 6b; Supplementary Fig. 6b). The gene discussed is TP53; the disease is esophageal squamous cell carcinoma.