However, ESCC tumors with higher Cycling program scores had fewer TMEM-CD8 cells, NMF, NAF1/2, CAF1, and NEC1/2 but more CAF2/3 and TEC2/3 as well as higher cytotoxicity, exhaustion, and Treg scores. This evidence concerns the gene NAF1 and esophageal squamous cell carcinoma.