To test the effectiveness of GEDI across neurodegenerative disease models, hSyn1:RGEDI-P2a-EGFP was cotransfected into rat cortical primary neurons with pGW1:HttEx1-Q25 or pGW1:HttEx1-Q97, pCAGGs:α-synuclein, or pGW1:TDP43 to generate previously characterized overexpression models of HD3, PD39, and ALS or FTD50, respectively (Fig. 3A). Here, HDAC3 is linked to neurodegenerative disease.