Combined treatment of 5-aza-dC with the histone deacetylase inhibitor trichostatin A restores WIF-1 expression, and restoration of WIF-1 inhibits colony formation, cellular proliferation, and anchorage-independent growth of TE-1 ESCC cells or SW48 colon cancer cells [60]. This evidence concerns the gene WIF1 and malignant colon neoplasm.