On the other hand, an increase was observed in the levels of activated CD4 non-Tregs (CD4 + CD25 + IL-10-TGF-β −) in PD patients after two years with respect to PD-0 yr patients (P = 0.019), and with respect to PD-1 yr (P = 0.014). This evidence concerns the gene TGFB1 and Parkinson disease.