We next accessed whether MARCH3 alternation could lead to the various degree of TIICs in HCC and found the CNV of MARCH3 occurred with arm-level gain could decrease the infiltrating levels of CD4 + T cells, macrophages and neutrophil cells, while MARCH3 alteration in high amplication could also decreased the infiltrating levels of B cells, CD4 + T cells, neutrophil cells and dendritic cells. The gene discussed is MARCHF3; the disease is hepatocellular carcinoma.