CD28 and cancer: In our study, advance cancer stage was negatively associated with levels of B cells, CD4+ T cells, naïve CD4+/CD4+, naïve CD4+ T cells, CD4+CD28+ T cells, CD8+CD28+ T cells and positively associated with NK cells, WBC counts, monocytes, neutrophils, eosinophils, basophils, MLR, NLR, ELR, BLR, PLR.