For instance, Palumbo et al. demonstrated an upregulated expression of UBE2C mRNA and protein in ESCC tissues through qRT-PCR and immunohistochemical analysis of ESCC and control tissue specimens and further confirmed, with in vitro experiments, that UBE2C affected the proliferation of ESCC cells by interfering with the level of cyclin B1 [4]. This evidence concerns the gene CCNB1 and esophageal squamous cell carcinoma.