Cortical spreading depression(CSD) is the electro-physiological correlate of migraine aura that causes opening of pannexin-1 megachannels and ATP release.CSD triggers parenchymal neurogenic inflammation(PNI) preceding meningeal inflammation which is the cause of migraine headache.P2X7-receptors are purinergic receptors activating pro-inflammatory cascades when extracellular ATP increased.We aim to investigate the effects of purinergic P2X7-receptors on CSD-induced PNI using a potent, selective and BBB permeable P2X7 antagonist(JNJ-47965567). This evidence concerns the gene P2RX7 and Headache.