To assess the role of microglia in Lyz2-DP1−/− mice, microglia were depleted by treatment with an inhibitor of CSF1R (colony-stimulating factor 1 receptor) (PLX5622) on the day of infection (group 1) or 3 days prior to infection (group 2), as depicted in Fig. 6a and e. Here, CSF1R is linked to infection.