Based on GEO datasets using prognoscan, we found high ACTB expression was correlated with poor OS (Fig. S2A), RFS (Fig. S2B), disease-specific survival (DSS) (Fig. S2C), distant metastasis free survival (DMFS) (Fig. S2D), distant recurrence free survival (DRFS) (Fig. S2E) and DFS (Fig. S2F) in various cancers (All P < 0.05). The gene discussed is ACTB; the disease is cancer.