In conclusion, the present study demonstrates that the inhibited expression of miR-25 inactivates the Wnt/β-catenin signaling pathway through targeting the DKK3 expression, thereby inhibiting proliferation of NPC cells, which contributes to the understanding of the molecular mechanism of miR-25 in the occurrence and development of NPC, and may provide a theoretical basis for the new treatment strategies for NPC. The gene discussed is DKK3; the disease is nasopharyngeal carcinoma.