Previous research on transgenic mice models supports our in silico analysis of PHPV to validate the function of these hub genes in hyaloid vasculature regression such as knockouts of the Arf tumor suppressor gene (23, 55, 56), p53 (21, 57), and Frizzled-5 (57) which were associated with PHPV-like phenotypes in mouse models. Here, FZD5 is linked to persistent hyperplastic primary vitreous.