PPARGC1A and Obesity: Although beyond the scope of this study, additional experiments involving whole genome methylation profiling, or assessment of methylation of genes such as PPARGC1A, IGFBP-1, and SLC6A4, which have previously been shown to be associated with T2D and obesity (Ling et al., 2008; Gu et al., 2013; Zhao et al., 2013), may be beneficial.