To assess whether GPR65 can mediate activation of AMPKα-regulated osteoclastogenic induction in CRC microenvironment, OCPs were induced into osteoclastogenic fate by stimulating with RANKL and M-CSF in the presence of CT-26 CM; meanwhile, plasmid containing GPR65 was transfected or cotransfected with AMPKα siRNA. Here, CSF1 is linked to colorectal carcinoma.