To investigate the molecular mechanisms by which NLGN3 promotes glioma cell proliferation and metastasis, we examined the key signaling pathway for proliferation, the PI3K-AKT, ERK1/2, and LYN pathway, as well as the markers for proliferation, Cyclin D1 and CDK4, as well as the markers for EMT progress, N-cad and E-cad using Western blot. This evidence concerns the gene CDH1 and glioma.