In addition, the expression of p-IRS2 and FOXO1 increased (p < 0.05, p < 0.0001, p < 0.001), and the expression of p-AKT decreased (p < 0.01) in the 15w-Z group, compared with the 15w-L group, confirming that insulin resistance appeared in the brain of 15-week-old ZDF rats (Figure 5C). Here, FOXO1 is linked to Insulin resistance.