We further validated these findings in xenograft tumor tissues using real-time q-PCR, which showed significant reduction in CD133 (fold change 0.41, p=0.002) and CD44 (fold change 0.77, p=0.05) mRNA levels in STIL-silenced tumor, which was further confirmed by IHC (Figures 4E–G), respectively. The gene discussed is PROM1; the disease is neoplasm.