TBX1 and neoplasm: In the present work, we identified three tumor-associated matricellular barriers: the first, with a localized increase in CAFs and a low deposition of Col V without obvious orientation near the malignant cells, the second with increased CAFs and Col I fibers aligned in parallel to the tumor border, and the third with increased CAFs and Col III bundled and aligned perpendicularly to the tumor border, suggesting that these Col types might be stronger malignant cells barrier compared to the fragile Col V counterparts.