When we assessed the distribution pattern of specific key TILs phenotypes, the first immune-cellular barrier coincided tumor portion exhibiting a higher presence of CD3+, PD-L1, LAG-3, CTLA-4, VISTA-1, macrophages, and NK cells, whereas the second immune-cellular barrier involved tumor intermediate portion with the presence of regulatory T cells (CD4+ and FOXP3+). This evidence concerns the gene LAG3 and neoplasm.