PBMCs and PMNLs were isolated from peripheral blood of NSCLC patients and live, CD66b+ Siglec8- cells were identified as LDNs and HDNs in the PBMC and PMNL fractions, respectively (Figure 2A, Siglec8 was used to separate neutrophils (Siglec8-) and eosinophils (Siglec8+) within the CD66b+ gate). Here, SIGLEC8 is linked to non-small cell lung carcinoma.