To investigate whether blockade of de-oligomerized SP-D in ALI attenuates ALI and lung inflammation in vivo, 10−12-week-old male C57BL/6 mice were treated with 0.4 mg i.t. or intraperitoneal (i.p.)aSP-D/kg mouse (sc-25324; Santa Cruz) in conjunction with 5 mg/kg LPS for 2 days (aSP-D i.t./LPS and aSP-D i.p./LPS groups). Here, SFTPD is linked to inflammatory response.