In the current paper, we have used Alt extract to induce airway inflammation in C57BL/6 and BALB/c mice, showing a different inflammatory response to Alt and observed that the induced asthma endotype is linked to different IL-33 processing in vivo. To study the relative contribution of neutrophils, anti-Ly6G neutralizing antibodies causing neutrophil depletion were injected into the mice, and inflammatory parameters along with cytokine profile were studied. The gene discussed is IL33; the disease is asthma.