MAPT and Alzheimer disease: Braak Staging, a well-established method that scores the accumulation of phospho-tau, is based on AT8 antibody staining (Braak and Braak, 1991; Braak et al., 2006); researchers have also identified the sequential appearance of specific tau phospho-dependent epitopes, revealing that AT100 phosphorylation appears after AT8 in human AD post-mortem brain tissue (Luna-Muñoz et al., 2007).