Under the pressure of ischemia and hypoxia after stroke, actin polymerization elicits stress fibers and concomitant endothelial cell contraction mediated by zipper-interacting protein kinase (ZIPK) through the phosphorylation of the myosin light chain (Vandenbroucke et al., 2008; Komarova and Malik, 2010; Shi et al., 2016; Zhang et al., 2019). The gene discussed is DAPK3; the disease is Stroke.