To investigate the correlation of autophagy at the invasive front with patient prognosis, we studied the interaction of SQSTM1 and LC3B in 148 NSCLC specimens, including 39 LUSC and 99 LUAD specimens, and in 10 tumor-adjacent lung tissues using bright-field PLA. The gene discussed is SQSTM1; the disease is non-small cell lung carcinoma.