Firstly, we found that the suppression of ALP staining caused by miR-508-3p mimic could be neutralized by SATB2 overexpression in OPLL cells (Figure 8D); And the expression of SATB2 (Supplementary Figure 3A, 3B) and osteogenesis-related markers (COL1, Runx2, OPN and OCN) (Figure 8E, 8F) suppressed by the miR-508-3p mimic could be rescued by SATB2 overexpression in OPLL cells. This evidence concerns the gene SPP1 and ossification of the posterior longitudinal ligament of the spine.