FAM171A1 and astrocytoma (excluding glioblastoma): This interaction could be verified both by co-localization seen with immunofluorescence of the endogenous proteins in human astrocytoma cells (U-373MG) and by co-immunoprecipitation followed by western blotting from lysates of COS-7 cells co-transfected with (cDNA)ADAM10 and (cDNA) APCN [15].