PTCH1 and neoplasm: In contrast, these key Hh pathway components were found to be significantly over-expressed in VSCC compared to normal vulval epithelium: SHH (median 1.0), PTCH1 (median 2.0), GLI1 (median 3.5) and GLI2 (median 3.5), and their expression were uniformly distributed across the tumour (Table 1).