Independent sample Kruskal Wallis test was applied for other Microparticles and immune cells; no significant differences of the distributions of TMP, PMP, and CD4+T cells across different stages of MM patients (p = 0.8, 0.6, 0.07 respectively), however significant difference in the distribution of PD1+CD4+T across ISS stages was detected (p = 0.041) as shown in Fig. 9. This evidence concerns the gene CD4 and Miyoshi myopathy.