ACE2 and infection: Similarly, the capacity of soluble ACE2 (sACE2) to neutralize pseudovirions bearing SARS-CoV-2 Spike D614G was significantly improved when preincubating the virus with sACE2 at 4 °C when compared with 37 °C prior infection of 293T-ACE2 target cells (Fig. 2, D and E).