Once purified botrocetin induces thrombocytopenia by a mechanism that is dependent on VWF, we investigated herein, using two experimental models, whether botrocetin and VWF are involved in BjV-induced thrombocytopenia, as the comprehension of these pathophysiological events may lead to better treatments of hemostatic disorders of patients bitten by B. jararaca snakes. Here, VWF is linked to vascular hemostatic disease.