At present, among relevant reports concerning primary liver cancer, most are the single detection of GP73, PIVKA-II, AFP-L3, AFP or other factors or the combined detection of 2 or 3 factors, and the combination of AFP and other serological indicators, including AFP+AFP-L3,15 AFP+PIVKA-II, AFP+GP73, AFP+AFP-L3+PIVKA-II.16,17 There are few related pieces of literature about MIF and primary liver cancer, and the direction of combined diagnosis of primary liver cancer by 5 related factors of MIF, GP73, PIVKA-II, AFP-L3, and AFP has not been clear yet. The gene discussed is AFP; the disease is liver cancer.