CXXC5 and breast adenocarcinoma: To ensure that the genetic fusion of 3F-CXXC5 to BirA*-HA does not affect the synthesis, the intracellular localization, and the biotinylation ability of the CXXC5-BirA* fusion protein, we initially carried out immunocytochemistry (ICC) and western blot (WB) analyses in transiently transfected MCF7 cells derived from a breast adenocarcinoma.