ROCK1 and ischemia: For instance, p-Drp1 (Ser616) was shown to promote transient global ischemia-induced neuronal damage, which was attenuated by Mdivi-1.53 Moreover, p-Drp1 (Ser616) levels were increased by nitric oxide, followed by the recruitment of p-Drp1 to mitochondria, thereby causing mitochondrial fragmentation.54 High glucose levels promoted ROCK1 activation and p-Drp1 (Ser616) expression, which are essential for mitochondrial fragmentation.55