Knock-in Drosophila lines were generated by CRISPR-Cas9 gene editing to explore how the R1648C (R-C) and R1648H (R-H) mutations in the SCN1A sodium channel gene contribute to distinct epilepsy disorders, Dravet syndrome, and GEFS+, respectively. This evidence concerns the gene SCN1A and encephalopathy, progressive, early-onset, with brain edema and/or leukoencephalopathy.