Taken together the results of these three databases, we observed and validated the significant (p<0.05) down-regulation of CXCL12 while significant (p<0.05) overexpression of CXCL12, CXCL8, AGT, GNB1, GNG4, and CXCL1 real hub genes at mRNA level in COAD patients of different clinicopathological features (patients race, cancer stages, genders, age groups, and body weight) relative to the normal controls. This evidence concerns the gene GNG4 and colon adenocarcinoma.