We performed pan‐cancer transcriptome analyses using TCGA data and found that ASF1B expression is significantly negatively associated with immune components of the TME in GBM and UCEC and negatively associated with stromal components of the TME in eight cancers, including BLCA, COAD, HNSC, LIHC, LUAD, OV, PAAD, and UCEC. Here, ASF1B is linked to cancer.