We found that WT BMDMs produced IFN-β in response to HSV1 (MOI, 10; 6 h post-infection) or F. novicida infection (MOI, 1; 3 h post-infection), while Aim2–/–, Asc–/– and Casp1–/– BMDMs did not (Extended Data Fig. 7e, f). This evidence concerns the gene AIM2 and infection.