Cytotoxicity correlated with upregulation of MHC-I and PD-L1 molecules on anti-PD-1-treated B16F10E-KO tumour cells as compared to isotype-treated B16F10E-KO (Supplementary Fig. 7c), which correlated with high production of IFNγ by CD8+ TIL (Supplementary Fig. 7d). The gene discussed is CD274; the disease is neoplasm.