ROC curve analysis of HCC biomarkers used in this study indicated that CTNNB1, SERPIND1 and SPINK1 have better diagnostic values with AUC values as 0.94 (95% CI = 0.87–1.01, p<0.0001), 0.88 (95% CI = 0.76–0.99, p = 0.0001) and 0.89 (95% CI = 0.77–1, p<0.0001), respectively, as shown in Fig 4. This evidence concerns the gene SERPIND1 and hepatocellular carcinoma.