The present study aimed to evaluate the effect of 5-Aza-CdR on p21Cip1/Waf1/Sdi1, p27Kip1, p57Kip2, and DNA methyltransferase 1genes expression, cell growth inhibition and cell apoptosis induction in colon cancer SW 480 and SW 948 cell lines. This evidence concerns the gene CDKN1A and malignant colon neoplasm.