In conclusion, our study has led to the identification of LCN2 as a new robust biomarker for predicting NEC development, which used in conjunction with CALPRO would have allowed the identification of VLNW infants more than a week before the diagnosis in four out of the eight cases that developed NEC, and in five out of the eight cases if PGE2 was also included in the algorithm. Here, LCN2 is linked to necrotizing enterocolitis.