LCN2 and necrotizing enterocolitis: As shown in Fig. 1 for CALPRO, LCN2, and HAPTO, variations in the levels of expression were seen for all three markers for both NEC and non-NEC samples but an apparent tendency for overexpression was noted for samples obtained from NEC infants (Fig. 1, left panels) and this tendency appeared to be relatively constant throughout the 10-day period preceding the diagnosis as confirmed by linear regression analysis (Fig. 1, black line in left panels).